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2ard

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The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination

Structural highlights

2ard is a 1 chain structure with sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2apg, 2aqj, 2ar8
Gene:	prnA (Pseudomonas fluorescens)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Chlorinated natural products include vancomycin and cryptophycin A. Their biosynthesis involves regioselective chlorination by flavin-dependent halogenases. We report the structural characterization of tryptophan 7-halogenase (PrnA), which regioselectively chlorinates tryptophan. Tryptophan and flavin adenine dinucleotide (FAD) are separated by a 10 angstrom-long tunnel and bound by distinct enzyme modules. The FAD module is conserved in halogenases and is related to flavin-dependent monooxygenases. On the basis of biochemical studies, crystal structures, and by analogy with monooxygenases, we predict that FADH2 reacts with O2 to make peroxyflavin, which is decomposed by Cl-. The resulting HOCl is guided through the tunnel to tryptophan, where it is activated to participate in electrophilic aromatic substitution.

Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination.,Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH Science. 2005 Sep 30;309(5744):2216-9. PMID:16195462^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH. Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination. Science. 2005 Sep 30;309(5744):2216-9. PMID:16195462 doi:309/5744/2216

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

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2ard

From Proteopedia

The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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