Despite the biological importance of self-splicing group II introns, little is known about their structural organization. Synthetic incorporation of site-specific photo-cross-linkers within catalytic domains resulted in functional distance constraints that, when combined with known tertiary interactions, provide a three-dimensional view of the active intron architecture. All functionalities important for both steps of splicing are proximal before the first step, suggestive of a single active-site region for group II intron catalysis.
A single active-site region for a group II intron.,de Lencastre A, Hamill S, Pyle AM Nat Struct Mol Biol. 2005 Jul;12(7):626-7. Epub 2005 Jun 26. PMID:15980867[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ de Lencastre A, Hamill S, Pyle AM. A single active-site region for a group II intron. Nat Struct Mol Biol. 2005 Jul;12(7):626-7. Epub 2005 Jun 26. PMID:15980867 doi:10.1038/nsmb957
