1iqp
From Proteopedia
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| , resolution 2.8Å | |||||||
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| Ligands: | |||||||
| Gene: | rfcs (Pyrococcus furiosus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Clamp Loader Small Subunit from Pyrococcus furiosus
Overview
In eukaryotic DNA replication, replication factor-C (RFC) acts as the clamp loader, which correctly installs the sliding clamp onto DNA strands at replication forks. The eukaryotic RFC is a complex consisting of one large and four small subunits. We have determined the crystal structure of the clamp loader small subunit (RFCS) from Pyrococcus furiosus. The six subunits, of which four bind ADP in their canonical nucleotide binding clefts, assemble into a dimer of semicircular trimers. The crescent-like architecture of each subunit formed by the three domains resembles that of the delta' subunit of the E. coli clamp loader. The trimeric architecture of archaeal RFCS, with its mobile N-terminal domains, involves intersubunit interactions that may be conserved in eukaryotic functional complexes.
About this Structure
1IQP is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.
Reference
Atomic structure of the clamp loader small subunit from Pyrococcus furiosus., Oyama T, Ishino Y, Cann IK, Ishino S, Morikawa K, Mol Cell. 2001 Aug;8(2):455-63. PMID:11545747
Page seeded by OCA on Thu Mar 20 11:53:39 2008
