2bgw
From Proteopedia
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XPF FROM AEROPYRUM PERNIX, COMPLEX WITH DNA
Overview
The XPF/Mus81 structure-specific endonucleases cleave double-stranded DNA, (dsDNA) within asymmetric branched DNA substrates and play an essential, role in nucleotide excision repair, recombination and genome integrity. We, report the structure of an archaeal XPF homodimer alone and bound to, dsDNA. Superposition of these structures reveals a large domain movement, upon binding DNA, indicating how the (HhH)(2) domain and the nuclease, domain are coupled to allow the recognition of, double-stranded/single-stranded DNA junctions. We identify two, nonequivalent DNA-binding sites and propose a model in which XPF distorts, the 3' flap substrate in order to engage both binding sites and promote, strand cleavage. The model rationalises published biochemical data and, implies a novel role for the ... [(full description)]
About this Structure
2BGW is a [Single protein] structure of sequence from [Aeropyrum pernix] with MG and SO4 as [ligands]. Full crystallographic information is available from [OCA].
Reference
Structure of an XPF endonuclease with and without DNA suggests a model for substrate recognition., Newman M, Murray-Rust J, Lally J, Rudolf J, Fadden A, Knowles PP, White MF, McDonald NQ, EMBO J. 2005 Mar 9;24(5):895-905. Epub 2005 Feb 17. PMID:15719018
Page seeded by OCA on Mon Oct 29 16:46:30 2007
