Publication Abstract from PubMed
Get3, Get4 and Get5 in Saccharomyces cerevisiae participate in the insertion of tail-anchored proteins into the endoplasmic reticulum membrane. We elucidated the interaction between Get4 and Get5 and investigated their interaction with Get3 and a tetratricopeptide repeat-containing protein, Sgt2. Based on co-immunoprecipitation and crystallographic studies, Get4 and Get5 formed a tight complex, suggesting that they constitute subunits of a larger complex. In contrast, although Get3 interacted physically with the Get4/Get5 complex, low amounts of Get3 co-precipitated with Get5, implying a transient interaction between Get3 and Get4/Get5. Sgt2 also interacted with Get5, although the amount of Sgt2 that co-precipitated with Get5 varied. Moreover, GET3, GET4 and GET5 interacted genetically with molecular chaperone YDJ1, suggesting that chaperones might also be involved in the insertion of tail-anchored proteins.
Crystal structure of Get4/Get5 complex and its interactions with Sgt2, Get3 and Ydj1.,Chang YW, Chuang YC, Ho YC, Cheng MY, Sun YJ, Hsiao CD, Wang C J Biol Chem. 2010 Jan 27. PMID:20106980[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
