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1eb4
From Proteopedia
Revision as of 08:59, 3 October 2014 by OCA (Talk | contribs)
1eb4 is a 1 chain structure with sequence from Pseudomonas putida. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Histidine ammonia-lyase requires a 4-methylidene-imidazole-5-one group (MIO) that is produced autocatalytically by a cyclization and dehydration step in a 3-residue loop of the polypeptide. The crystal structures of three mutants have been established. Two mutants were inactive and failed to form MIO, but remained unchanged elsewhere. The third mutant showed very low activity and formed MIO, although it differed from an MIO-less mutant only by an additional 329-C(beta) atom. This atom forms one constraint during MIO formation, the other being the strongly connected Asp145. An exploration of the conformational space of the MIO-forming loop showed that the cyclization is probably enforced by a mechanic compression in a late stage of chain folding and is catalyzed by a well-connected internal water molecule. The cyclization of the respective 3-residue loop of green fluorescent protein is likely to occur in a similar reaction.
Autocatalytic peptide cyclization during chain folding of histidine ammonia-lyase.,Baedeker M, Schulz GE Structure. 2002 Jan;10(1):61-7. PMID:11796111[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Baedeker M, Schulz GE. Autocatalytic peptide cyclization during chain folding of histidine ammonia-lyase. Structure. 2002 Jan;10(1):61-7. PMID:11796111
