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spinqualitylabelsall models PDB ID 1jqs Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
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Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog

Overview

L11 protein is located at the base of the L7/L12 stalk of the 50 S subunit of the Escherichia coli ribosome. Because of the flexible nature of the region, recent X-ray crystallographic studies of the 50 S subunit failed to locate the N-terminal domain of the protein. We have determined the position of the complete L11 protein by comparing a three-dimensional cryo-EM reconstruction of the 70 S ribosome, isolated from a mutant lacking ribosomal protein L11, with the three-dimensional map of the wild-type ribosome. Fitting of the X-ray coordinates of L11-23 S RNA complex and EF-G into the cryo-EM maps combined with molecular modeling, reveals that, following EF-G-dependent GTP hydrolysis, domain V of EF-G intrudes into the cleft between the 23 S ribosomal RNA and the N-terminal domain of L11 (where the antibiotic thiostrepton binds), causing the N-terminal domain to move and thereby inducing the formation of the arc-like connection with the G' domain of EF-G. The results provide a new insight into the mechanism of EF-G-dependent translocation.

About this Structure

1JQS is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation., Agrawal RK, Linde J, Sengupta J, Nierhaus KH, Frank J, J Mol Biol. 2001 Aug 24;311(4):777-87. PMID:11518530

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