| Structural highlights
2xqd is a 26 chain structure with sequence from Escherichia coli and Thermus thermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , , , |
| NonStd Res: | , , , , , , |
| Related: | 2xqe, 1dk1, 1ibk, 1f7y, 1lou, 1ibl, 2uuc, 2wdk, 1kuq, 2c77, 1ab3, 2j02, 1qjh, 1j5e, 2wrq, 2b9o, 2j00, 1hnx, 2x9t, 2wh3, 2uub, 1mvr, 1cqn, 1emw, 2v46, 1pnx, 1qkf, 1qkh, 2vqf, 2wh1, 1n32, 2wrn, 2jl5, 2f4v, 1twt, 2uxd, 1fka, 1n36, 2uxc, 1fjg, 1hnw, 1l1u, 1hr0, 2v48, 1an7, 2b64, 1xmq, 2b9m, 2wdg, 1xnr, 1hnz, 2wdh, 1jgq, 1i94, 1cqm, 1i96, 2uu9, 1eg0, 1vox, 1ibm, 2uxb, 2vqe, 2bvz, 1i95, 2uua, 1voz, 1xnq, 2xfz, 2bxj, 1qd7, 1gix, 1jgo, 1jgp, 2xg1, 2x9r, 1n34, 1n33, 1pns, 2jl7, 1yl4, 1ris, 2wdm, 1xmo, 1i97 |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Publication Abstract from PubMed
Protein synthesis requires several guanosine triphosphatase (GTPase) factors, including elongation factor Tu (EF-Tu), which delivers aminoacyl-transfer RNAs (tRNAs) to the ribosome. To understand how the ribosome triggers GTP hydrolysis in translational GTPases, we have determined the crystal structure of EF-Tu and aminoacyl-tRNA bound to the ribosome with a GTP analog, to 3.2 angstrom resolution. EF-Tu is in its active conformation, the switch I loop is ordered, and the catalytic histidine is coordinating the nucleophilic water in position for inline attack on the gamma-phosphate of GTP. This activated conformation is due to a critical and conserved interaction of the histidine with A2662 of the sarcin-ricin loop of the 23S ribosomal RNA. The structure suggests a universal mechanism for GTPase activation and hydrolysis in translational GTPases on the ribosome.
The mechanism for activation of GTP hydrolysis on the ribosome.,Voorhees RM, Schmeing TM, Kelley AC, Ramakrishnan V Science. 2010 Nov 5;330(6005):835-8. PMID:21051640[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Voorhees RM, Schmeing TM, Kelley AC, Ramakrishnan V. The mechanism for activation of GTP hydrolysis on the ribosome. Science. 2010 Nov 5;330(6005):835-8. PMID:21051640 doi:10.1126/science.1194460
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