Carbohydrate-binding domain modules(CBM) are non-catalytic domain which present in various microrganisms. [1]
Function
Carbohydrate-binding Module
The most important conformational element of most CBMs is the . t. The folds and architecture displayed by these beta-sheets have been classified into seven families with the β-sandwich being the most recurrent fold and the fold of family 3. [2]
Applications
The cellulose-binding module (CBM) is an attractive affinity tag for protein purification for several reasons:
1.Very specific binding ability to the fused protein eith CBM tag.
2.Very low nonspecific binding ability to to other protein.
3.efficient release of bound protein under nondenaturing conditions. [3]
Relevance
Structural highlights
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