1lv0
From Proteopedia
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| , resolution 2.00Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Rab effector guanine nucleotide dissociation inhibitor (GDI) in complex with a geranylgeranyl (GG) peptide
Overview
Rab GTPases, key regulators of membrane targeting and fusion, require the covalent attachment of geranylgeranyl lipids to their C terminus for function. To elucidate the role of lipid in Rab recycling, we have determined the crystal structure of Rab guanine nucleotide dissociation inhibitor (alphaGDI) in complex with a geranylgeranyl (GG) ligand (H(2)N-Cys-(S-GG)-OMe). The lipid is bound beneath the Rab binding platform in a shallow hydrophobic groove. Mutation of the binding pocket in the brain-specific alphaGDI leads to mental retardation. Strikingly, lipid binding acts through a conserved allosteric switching mechanism to promote release of the GDI-Rab[GDP] complex from the membrane.
About this Structure
1LV0 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Geranylgeranyl switching regulates GDI-Rab GTPase recycling., An Y, Shao Y, Alory C, Matteson J, Sakisaka T, Chen W, Gibbs RA, Wilson IA, Balch WE, Structure. 2003 Mar;11(3):347-57. PMID:12623022
Page seeded by OCA on Thu Mar 20 12:35:31 2008
Categories: Bos taurus | Single protein | Alory, C. | An, Y. | Balch, W E. | Chen, W. | Gibbs, R A. | Matteson, J. | Sakisaka, T. | Shao, Y. | Wilson, I A. | GER | SO4 | Protein-ligand complex
