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Cas9

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Revision as of 20:43, 30 November 2014 by Wayne Decatur (Talk | contribs)
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Cas9 is the RNA-guided DNA endonuclease used by the CRISPR(clustered regularly interspaced short palindromic repeats)-associated systems to generate double-strand DNA breaks in the invading DNA during an adaptive bacterial immune response.

It has been exploited for use in genome editing systems.

Articles in Proteopedia concerning Cas9 include:

  • any?

Contents

3D structures of Cas9

Streptococcus pyogenes Cas9

  • 4un3, 4un4, and 4un5 - S. pyogenes Cas9 bound to sgRNA and target DNA
  • 4oo8 - - S. pyogenes Cas9 bound to sgRNA and target DNA
  • 4cmp
  • 4cmq - Mn2+-bound S. pyogenes Cas9

Actinomyces naeslundii Cas9

  • 4oge
  • 4ogc - Mn2+-bound A.s naeslundii Cas9

See Also

HNH endonuclease


References

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