1n11

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spinqualitylabelsall models PDB ID 1n11 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
, resolution 2.70Å
Ligands:	and
Gene:	ank-1 (Homo sapiens)
Coordinates:	save as pdb, mmCIF, xml

D34 REGION OF HUMAN ANKYRIN-R AND LINKER

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Ankyrins are multifunctional adaptors that link specific proteins to the membrane-associated, spectrin- actin cytoskeleton. The N-terminal, 'membrane-binding' domain of ankyrins contains 24 ANK repeats and mediates most binding activities. Repeats 13-24 are especially active, with known sites of interaction for the Na/K ATPase, Cl/HCO(3) anion exchanger, voltage-gated sodium channel, clathrin heavy chain and L1 family cell adhesion molecules. Here we report the crystal structure of a human ankyrinR construct containing ANK repeats 13-24 and a portion of the spectrin-binding domain. The ANK repeats are observed to form a contiguous spiral stack with which the spectrin-binding domain fragment associates as an extended strand. The structural information has been used to construct models of all 24 repeats of the membrane-binding domain as well as the interactions of the repeats with the Cl/HCO(3) anion exchanger and clathrin. These models, together with available binding studies, suggest that ion transporters such as the anion exchanger associate in a large central cavity formed by the ANK repeat spiral, while clathrin and cell adhesion molecules associate with specific regions outside this cavity.

Disease

Known diseases associated with this structure: Chondrocalcinosis 2 OMIM:[605145], Craniometaphyseal dysplasia OMIM:[605145]

About this Structure

1N11 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of a 12 ANK repeat stack from human ankyrinR., Michaely P, Tomchick DR, Machius M, Anderson RG, EMBO J. 2002 Dec 2;21(23):6387-96. PMID:12456646

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