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1njf
From Proteopedia
| |||||||
| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | DnaX (Escherichia coli) | ||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Nucleotide bound form of an isolated E. coli clamp loader gamma subunit
Overview
Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes. The structure of the E. coli clamp loader in a nucleotide-free state has been determined previously. We now report crystal structures of a truncated form of the isolated gamma-ATPase subunit, gamma(1-243), of the E. coli clamp loader, in nucleotide-free and bound forms. The gamma subunit adopts a defined conformation when empty, in which the nucleotide binding site is blocked. The binding of either ATPgammaS or ADP, which are shown to bind with equal affinity to gamma(1-243), induces a change in the relative orientation of the two domains such that nucleotides can be accommodated. This change would break one of the gamma:gamma interfaces seen in the empty clamp loader complex, and may represent one step in the activation process.
About this Structure
1NJF is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit., Podobnik M, Weitze TF, O'Donnell M, Kuriyan J, Structure. 2003 Mar;11(3):253-63. PMID:12623013
Page seeded by OCA on Thu Mar 20 12:57:41 2008
