1om2
From Proteopedia
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| Activity: | Aldehyde dehydrogenase (NAD(+)), with EC number 1.2.1.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION NMR STRUCTURE OF THE MITOCHONDRIAL PROTEIN IMPORT RECEPTOR TOM20 FROM RAT IN A COMPLEX WITH A PRESEQUENCE PEPTIDE DERIVED FROM RAT ALDEHYDE DEHYDROGENASE (ALDH)
Overview
Most mitochondrial proteins are synthesized in the cytosol as precursor proteins with a cleavable N-terminal presequence and are imported into mitochondria. We report here the NMR structure of a general import receptor, rat Tom20, in a complex with a presequence peptide derived from rat aldehyde dehydrogenase. The cytosolic domain of Tom20 forms an all alpha-helical structure with a groove to accommodate the presequence peptide. The bound presequence forms an amphiphilic helical structure with hydrophobic leucines aligned on one side to interact with a hydrophobic patch in the Tom20 groove. Although the positive charges of the presequence are essential for import ability, presequence binding to Tom20 is mediated mainly by hydrophobic rather than ionic interactions.
About this Structure
1OM2 is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20., Abe Y, Shodai T, Muto T, Mihara K, Torii H, Nishikawa S, Endo T, Kohda D, Cell. 2000 Mar 3;100(5):551-60. PMID:10721992
Page seeded by OCA on Thu Mar 20 13:12:37 2008
Categories: Aldehyde dehydrogenase (NAD(+)) | Protein complex | Rattus norvegicus | Abe, Y. | Endo, T. | Kohda, D. | Mihara, K. | Muto, T. | Nishikawa, S. | Shodai, T. | Torii, H. | Mitochondrial protein import across outer membrane | Mitochondrial targeting signal | Presequence peptide | Receptor for presequence
