1q1h
From Proteopedia
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| , resolution 2.90Å | |||||||
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| Gene: | tfE (Sulfolobus solfataricus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
An extended winged helix domain in general transcription factor E/IIE alpha
Overview
Initiation of eukaryotic mRNA transcription requires melting of promoter DNA with the help of the general transcription factors TFIIE and TFIIH. Here we define a conserved and functionally essential N-terminal domain in TFE, the archaeal homolog of the large TFIIE subunit alpha. X-ray crystallography shows that this TFE domain adopts a winged helix-turn-helix (winged helix) fold, extended by specific alpha-helices at the N and C termini. Although the winged helix fold is often found in DNA-binding proteins, we show that TFE is not a typical DNA-binding winged helix protein, because its putative DNA-binding face shows a negatively charged groove and an unusually long wing, and because the domain lacks DNA-binding activity in vitro. The groove and a conserved hydrophobic surface patch on the additional N-terminal alpha-helix may, however, allow for interactions with other general transcription factors and RNA polymerase. Homology modeling shows that the TFE domain is conserved in TFIIE alpha, including the potential functional surfaces.
About this Structure
1Q1H is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
An extended winged helix domain in general transcription factor E/IIE alpha., Meinhart A, Blobel J, Cramer P, J Biol Chem. 2003 Nov 28;278(48):48267-74. Epub 2003 Sep 17. PMID:13679366
Page seeded by OCA on Thu Mar 20 13:31:48 2008
