1rxe
From Proteopedia
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| , resolution 1.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | ARSC, SAP018 (Staphylococcus aureus) | ||||||
| Activity: | Arsenate reductase (glutaredoxin), with EC number 1.20.4.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ArsC complexed with MNB
Overview
Structural insights into formation of the complex between the ubiquitous thiol-disulfide oxidoreductase thioredoxin and its oxidized substrate are under-documented owing to its entropical instability. In vitro, it is possible via a reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) to make a stable mixed-disulfide complex between thioredoxin from Staphylococcus aureus and one of its substrates, oxidized pI258 arsenate reductase (ArsC) from S. aureus. In the absence of the crystal structure of an ArsC-thioredoxin complex, the structures of two precursors of the complex, the ArsC triple mutant ArsC C10SC15AC82S and its 5-thio-2-nitrobenzoic acid (TNB) adduct, were determined. The ArsC triple mutant has a structure very similar to that of the reduced form of wild-type ArsC, with a folded redox helix and a buried catalytic Cys89. In the adduct form, the TNB molecule is buried in a hydrophobic pocket and the disulfide bridge between TNB and Cys89 is sterically inaccessible to thioredoxin. In order to form a mixed disulfide between ArsC and thioredoxin, a change in the orientation of the TNB-Cys89 disulfide in the structure is necessary.
About this Structure
1RXE is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
The structure of a triple mutant of pI258 arsenate reductase from Staphylococcus aureus and its 5-thio-2-nitrobenzoic acid adduct., Messens J, Van Molle I, Vanhaesebrouck P, Van Belle K, Wahni K, Martins JC, Wyns L, Loris R, Acta Crystallogr D Biol Crystallogr. 2004 Jun;60(Pt 6):1180-4. Epub 2004, May 21. PMID:15159594
Page seeded by OCA on Thu Mar 20 13:57:30 2008
Categories: Arsenate reductase (glutaredoxin) | Single protein | Staphylococcus aureus | Belle, K Van. | Limbourg, M. | Loris, R. | Martins, J C. | Messens, J. | Molle, I Van. | Vanhaesebrouck, P. | Wahni, K. | Wyns, L. | K | LCP | MNB | 5-mercapto-2-nitrobenzoic acid | 5-thio-2-nitrobenzoic acid | Arsc | Mixed disulphide | Mnb | Tnb
