1st8
From Proteopedia
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| , resolution 2.35Å | |||||||
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| Ligands: | |||||||
| Gene: | 1-feh IIa (Cichorium intybus) | ||||||
| Activity: | Fructan beta-fructosidase, with EC number 3.2.1.80 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus
Overview
Fructan 1-exohydrolase, an enzyme involved in fructan degradation, belongs to the glycosyl hydrolase family 32. The structure of isoenzyme 1-FEH IIa from Cichorium intybus is described at a resolution of 2.35 A. The structure consists of an N-terminal fivefold beta-propeller domain connected to two C-terminal beta-sheets. The putative active site is located entirely in the beta-propeller domain and is formed by amino acids which are highly conserved within glycosyl hydrolase family 32. The fructan-binding site is thought to be in the cleft formed between the two domains. The 1-FEH IIa structure is compared with the structures of two homologous but functionally different enzymes: a levansucrase from Bacillus subtilis (glycosyl hydrolase family 68) and an invertase from Thermotoga maritima (glycosyl hydrolase family 32).
About this Structure
1ST8 is a Single protein structure of sequence from Cichorium intybus. Full crystallographic information is available from OCA.
Reference
X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus., Verhaest M, Ende WV, Roy KL, De Ranter CJ, Laere AV, Rabijns A, Plant J. 2005 Feb;41(3):400-11. PMID:15659099
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