Structural highlights
Publication Abstract from PubMed
The crystal structure of [d(CGCAAATTTGCG)]2 has been determined to 1.5 A resolution, representing the first high-resolution structure of this DNA fragment. The ion interactions are novel. A spermine molecule replaces a Mg2+ observed in analogous structures. Unlike lower-resolution structures, the minor groove is narrow and the major groove lacks extra Watson-Crick hydrogen bonds. In addition, a monolayer of solvent sites, including a "spine of hydration", is visible in the minor groove. The crystal of [d(CGCAAATTTGCG)]2 was grown from a solution containing spermine, magnesium, and lithium. The conformation recapitulates that of "monovalent-minus" DNA.
High-resolution structure of an extended A-tract: [d(CGCAAATTTGCG)]2.,Woods KK, Maehigashi T, Howerton SB, Sines CC, Tannenbaum S, Williams LD J Am Chem Soc. 2004 Dec 1;126(47):15330-1. PMID:15563130[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Woods KK, Maehigashi T, Howerton SB, Sines CC, Tannenbaum S, Williams LD. High-resolution structure of an extended A-tract: [d(CGCAAATTTGCG)]2. J Am Chem Soc. 2004 Dec 1;126(47):15330-1. PMID:15563130 doi:10.1021/ja045207x
