1udi
From Proteopedia
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| , resolution 2.7Å | |||||||
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| Activity: | Uridine nucleosidase, with EC number 3.2.2.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NUCLEOTIDE MIMICRY IN THE CRYSTAL STRUCTURE OF THE URACIL-DNA GLYCOSYLASE-URACIL GLYCOSYLASE INHIBITOR PROTEIN COMPLEX
Overview
The Bacillus subtilis bacteriophages PBS-1 and PBS-2 protect their uracil-containing DNA by expressing an inhibitor protein (UGI) which inactivates the host uracil-DNA glycosylase (UDGase) base-excision repair enzyme. Also, PBS1/2 UGI efficiently inactivates UDGases from other biological sources, including the enzyme from herpes simplex virus type-1 (HSV-1). The crystal structure of the HSV-1 UDGase-PBS1 UGI complex at 2.7 angstrum reveals an alpha-beta-alpha sandwich structure for UGI which interacts with conserved regions of UDGase involved in DNA binding, and directly mimics protein-DNA interactions observed in the UDGase-oligonucleotide complex. The inhibitor completely blocks access to the active site of UDGase, but makes no direct contact with the uracil-binding pocket itself.
About this Structure
1UDI is a Protein complex structure of sequences from Human herpesvirus 4 and Phage pbs1. Full crystallographic information is available from OCA.
Reference
Nucleotide mimicry in the crystal structure of the uracil-DNA glycosylase-uracil glycosylase inhibitor protein complex., Savva R, Pearl LH, Nat Struct Biol. 1995 Sep;2(9):752-7. PMID:7552746
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