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1vbk

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Revision as of 12:43, 20 March 2008 by OCA (Talk | contribs)
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PDB ID 1vbk

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, resolution 1.90Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



Crystal structure of PH1313 from Pyrococcus horikoshii Ot3


Overview

The putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3 has been overexpressed and purified. Crystallization was performed by the oil-microbatch method using 28%(v/v) 2-methyl-2,4-pentanediol as a precipitant at 291 K. A native X-ray diffraction data set at 1.9 A resolution and a single anomalous dispersion data set from a selenomethionine-derivative crystal at 2.1 A resolution were collected using synchrotron radiation at 100 K. The native crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 71.7, b = 71.2, c = 141.8 A.

About this Structure

1VBK is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.

Reference

Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3., Sugahara M, Murai S, Sugahara M, Kunishima N, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jan 1;63(Pt, 1):56-8. Epub 2006 Dec 22. PMID:17183176

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