1w63
From Proteopedia
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AP1 CLATHRIN ADAPTOR CORE
Overview
The heterotetrameric adaptor proteins (AP complexes) link the outer lattice of clathrin-coated vesicles with membrane-anchored cargo molecules. We report the crystal structure of the core of the AP-1 complex, which functions in the trans-Golgi network (TGN). Packing of complexes in the crystal generates an exceptionally long (1,135-A) unit-cell axis, but the 6-fold noncrystallographic redundancy yields an excellent map at 4-A resolution. The AP-1 core comprises N-terminal fragments of the two large chains, beta1 and gamma, and the intact medium and small chains, micro1 and sigma1. Its molecular architecture closely resembles that of the core of AP-2, the plasma-membrane-specific adaptor, for which a structure has been determined. Both structures represent an "inactive" conformation with respect to binding of cargo with a tyrosine-based sorting signal. TGN localization of AP-1 depends on the small GTPase, Arf1, and the phosphoinositide, PI-4-P. We show that directed mutations of residues at a particular corner of the gamma chain prevent recruitment to the TGN in cells and diminish PI-4-P-dependent, but not Arf1-dependent, liposome binding in vitro.
About this Structure
1W63 is a Protein complex structure of sequences from Mus musculus and Rattus norvegicus. The following page contains interesting information on the relation of 1W63 with [Clathrin]. Full crystallographic information is available from OCA.
Reference
Crystal structure of the clathrin adaptor protein 1 core., Heldwein EE, Macia E, Wang J, Yin HL, Kirchhausen T, Harrison SC, Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14108-13. Epub 2004 Sep 17. PMID:15377783
Page seeded by OCA on Thu Mar 20 14:52:43 2008
