1wo5

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spinqualitylabelsall models PDB ID 1wo5 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
Ligands:
Activity:	Histone acetyltransferase, with EC number 2.3.1.48
Coordinates:	save as pdb, mmCIF, xml

Solution structure of Designed Functional Finger 2 (DFF2): Designed mutant based on non-native CHANCE domain

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Zinc binding motifs have received much attention in the area of protein design. Here, we have tested the suitability of a recently discovered nonnative zinc binding structure as a protein design scaffold. A series of multiple alanine mutants was created to investigate the minimal requirements for folding, and solution structures of these mutants showed that the original fold was maintained, despite changes in approximately 50% of the sequence. We next attempted to transplant binding faces from chosen bimolecular interactions onto one of these mutants, and many of the resulting "chimeras" were shown to adopt a native-like fold. These results both highlight the robust nature of small zinc binding domains and underscore the complexity of designing functional proteins, even using such small, highly ordered scaffolds as templates.

Disease

Known diseases associated with this structure: Blue-cone monochromacy OMIM:[303900], Colorblindness, protan OMIM:[303900], Rubenstein-Taybi syndrome OMIM:[600140]

About this Structure

1WO5 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Assessment of the robustness of a serendipitous zinc binding fold: mutagenesis and protein grafting., Sharpe BK, Liew CK, Kwan AH, Wilce JA, Crossley M, Matthews JM, Mackay JP, Structure. 2005 Feb;13(2):257-66. PMID:15698569

Page seeded by OCA on Thu Mar 20 14:59:28 2008