4nc9

Structural highlights

Function

[PIMA_MYCS2] Catalyzes the addition of a mannose residue from GDP-D-mannose to the position 2 of a phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Contrary to PimB, the mannosyltransferase PimA is unable to transfer a mannose residue to the position 6 of the phosphatidyl-myo-inositola of PIM1.^{[1] [2]}

References

1. ↑ Kordulakova J, Gilleron M, Mikusova K, Puzo G, Brennan PJ, Gicquel B, Jackson M. Definition of the first mannosylation step in phosphatidylinositol mannoside synthesis. PimA is essential for growth of mycobacteria. J Biol Chem. 2002 Aug 30;277(35):31335-44. Epub 2002 Jun 14. PMID:12068013 doi:10.1074/jbc.M204060200
2. ↑ Guerin ME, Kaur D, Somashekar BS, Gibbs S, Gest P, Chatterjee D, Brennan PJ, Jackson M. New insights into the early steps of phosphatidylinositol mannoside biosynthesis in mycobacteria: PimB' is an essential enzyme of Mycobacterium smegmatis. J Biol Chem. 2009 Sep 18;284(38):25687-96. doi: 10.1074/jbc.M109.030593. Epub, 2009 Jul 28. PMID:19638342 doi:http://dx.doi.org/10.1074/jbc.M109.030593