| Structural highlights
Function
[CHIC_SECCE] Defense against chitin containing fungal pathogens. Binds the hyphal tips of fungi and degrades nascent chitin.[1] [2] [3]
Publication Abstract from PubMed
Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)(4) revealed that the entire substrate-binding cleft was completely occupied with the sugar residues of two (GlcNAc)(4) molecules. One (GlcNAc)(4) molecule bound to subsites -4 to -1, while the other bound to subsites +1 to +4. Comparisons of the main chain conformation between liganded RSC-c-E67Q/W72A and unliganded wild type RSC-c suggested domain motion essential for catalysis. This is the first report on the complete subsite mapping of GH19 chitinase.
Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure.,Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T FEBS Lett. 2013 Aug 19;587(16):2691-7. doi: 10.1016/j.febslet.2013.07.008. Epub, 2013 Jul 18. PMID:23871710[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ohnuma T, Yagi M, Yamagami T, Taira T, Aso Y, Ishiguro M. Molecular cloning, functional expression, and mutagenesis of cDNA encoding rye (Secale cereale) seed chitinase-c. Biosci Biotechnol Biochem. 2002 Feb;66(2):277-84. PMID:11999399
- ↑ Taira T, Yamagami T, Aso Y, Ishiguro M, Ishihara M. Localization, accumulation, and antifungal activity of chitinases in rye (Secale cereale) seed. Biosci Biotechnol Biochem. 2001 Dec;65(12):2710-8. PMID:11826968
- ↑ Taira T, Ohnuma T, Yamagami T, Aso Y, Ishiguro M, Ishihara M. Antifungal activity of rye (Secale cereale) seed chitinases: the different binding manner of class I and class II chitinases to the fungal cell walls. Biosci Biotechnol Biochem. 2002 May;66(5):970-7. PMID:12092848
- ↑ Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T. Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure. FEBS Lett. 2013 Aug 19;587(16):2691-7. doi: 10.1016/j.febslet.2013.07.008. Epub, 2013 Jul 18. PMID:23871710 doi:http://dx.doi.org/10.1016/j.febslet.2013.07.008
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