Structural highlights
Function
[PDR16_YEAST] Has phosphatidylinositol transfer activity. Involved in the regulation of the phospholipid composition of plasma- and endomembranes. Altering plasma membrane composition may provide a possible mechanism for multidrug resistance. Involved in the regulation of sterol biosynthesis. Contributes to efficient phospholipase D1 activation in the regulation of phospholipid turnover.[1] [2]
Publication Abstract from PubMed
Phosphorylated derivatives of phosphatidylinositol (PtdIns), also called phosphoinositides (PIPs), are basic components of membrane-associated signalling systems. A family of PtdIns-transfer proteins (PITPs) called the Sec14 family have been predicted to form a set of functional modules that can sense different types of lipid metabolism and transmit the information to the PIP signalling system. In eukaryotic cells, the Sec14 family exhibits a wide diversity of activity, but the structural basis of this diversity remains unclear. In the present study, the dimeric structure of Sfh3 (Sec14 family homologue 3 in yeast) is reported for the first time and differs from the Sec14 proteins reported to date, all of which are monomeric. Some variations in the binding pocket of Sfh3 were observed and the dimer interface was identified and proposed to provide a link between dimer-monomer state changes and PtdIns binding. Together, these structural changes and the oligomeric state transformation of Sfh3 support ideas of diversity within the Sec14 family and provide some new clues to function.
Dimeric Sfh3 has structural changes in its binding pocket that are associated with a dimer-monomer state transformation induced by substrate binding.,Yuan Y, Zhao W, Wang X, Gao Y, Niu L, Teng M Acta Crystallogr D Biol Crystallogr. 2013 Mar;69(Pt 3):313-23. doi:, 10.1107/S0907444912046161. Epub 2013 Feb 16. PMID:23519406[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ van den Hazel HB, Pichler H, do Valle Matta MA, Leitner E, Goffeau A, Daum G. PDR16 and PDR17, two homologous genes of Saccharomyces cerevisiae, affect lipid biosynthesis and resistance to multiple drugs. J Biol Chem. 1999 Jan 22;274(4):1934-41. PMID:9890948
- ↑ Li X, Routt SM, Xie Z, Cui X, Fang M, Kearns MA, Bard M, Kirsch DR, Bankaitis VA. Identification of a novel family of nonclassic yeast phosphatidylinositol transfer proteins whose function modulates phospholipase D activity and Sec14p-independent cell growth. Mol Biol Cell. 2000 Jun;11(6):1989-2005. PMID:10848624
- ↑ Yuan Y, Zhao W, Wang X, Gao Y, Niu L, Teng M. Dimeric Sfh3 has structural changes in its binding pocket that are associated with a dimer-monomer state transformation induced by substrate binding. Acta Crystallogr D Biol Crystallogr. 2013 Mar;69(Pt 3):313-23. doi:, 10.1107/S0907444912046161. Epub 2013 Feb 16. PMID:23519406 doi:10.1107/S0907444912046161
