| Structural highlights
Function
[CLASP_DROME] Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for several aspects of mitotic spindle formation including the formation of the overlapping central spindle microtubules and kinetochore attachment. Required for the incorporation of tubulin subunits at the plus ends of kinetochore microtubules during poleward microtubule flux. Acts antagonistically to Klp10A and Klp67A to maintain metaphase spindle length. Also required for guidance of CNS axons downstream of Abl. May function to identify a subset of microtubules that probe the peripheral growth cone domain, where guidance signals exert their influence on cytoskeletal organization. Also required during oogenesis for the organization of the polarized microtubule network inside the 16-cell cyst that ensures oocyte differentiation.[1] [2] [3] [4] [5] [6] [7] [8] [9]
Publication Abstract from PubMed
Mast/Orbit is a nonmotor microtubule-associated protein (MAP) present in Drosophila melanogaster that reportedly binds microtubules at the plus end and is essential for mitosis. Sequence analysis has shown that the N-terminal domain (Mast-M1) resembles TOG domains from the Dis1-TOG family of proteins and stands as a representative of one of the three subclasses of divergent TOG-like domains (TOGL1) that includes human CLASP1. The crystal structure of Mast-M1 has been determined at 2.0 A resolution and provides the first detailed structural description of any TOG-like domain. The structure confirms that Mast-M1 adopts a similar fold to the previously described Dis1-TOG domains of microtubule-binding proteins. A comparison with three known TOG-domain structures from XMAP215/Dis1 family members exposes significant differences between Mast-M1 and other TOG-domain structures in key residues at the proposed tubulin-binding edge.
The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit.,De la Mora-Rey T, Guenther BD, Finzel BC Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jul 1;69(Pt 7):723-9. doi:, 10.1107/S1744309113015182. Epub 2013 Jun 27. PMID:23832196[10]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lemos CL, Sampaio P, Maiato H, Costa M, Omel'yanchuk LV, Liberal V, Sunkel CE. Mast, a conserved microtubule-associated protein required for bipolar mitotic spindle organization. EMBO J. 2000 Jul 17;19(14):3668-82. PMID:10899121 doi:10.1093/emboj/19.14.3668
- ↑ Inoue YH, do Carmo Avides M, Shiraki M, Deak P, Yamaguchi M, Nishimoto Y, Matsukage A, Glover DM. Orbit, a novel microtubule-associated protein essential for mitosis in Drosophila melanogaster. J Cell Biol. 2000 Apr 3;149(1):153-66. PMID:10747094
- ↑ Maiato H, Sampaio P, Lemos CL, Findlay J, Carmena M, Earnshaw WC, Sunkel CE. MAST/Orbit has a role in microtubule-kinetochore attachment and is essential for chromosome alignment and maintenance of spindle bipolarity. J Cell Biol. 2002 May 27;157(5):749-60. Epub 2002 May 28. PMID:12034769 doi:10.1083/jcb.200201101
- ↑ Mathe E, Inoue YH, Palframan W, Brown G, Glover DM. Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis. Development. 2003 Mar;130(5):901-15. PMID:12538517
- ↑ Inoue YH, Savoian MS, Suzuki T, Mathe E, Yamamoto MT, Glover DM. Mutations in orbit/mast reveal that the central spindle is comprised of two microtubule populations, those that initiate cleavage and those that propagate furrow ingression. J Cell Biol. 2004 Jul 5;166(1):49-60. PMID:15240569 doi:10.1083/jcb.200402052
- ↑ Lee H, Engel U, Rusch J, Scherrer S, Sheard K, Van Vactor D. The microtubule plus end tracking protein Orbit/MAST/CLASP acts downstream of the tyrosine kinase Abl in mediating axon guidance. Neuron. 2004 Jun 24;42(6):913-26. PMID:15207236 doi:10.1016/j.neuron.2004.05.020
- ↑ Goshima G, Wollman R, Stuurman N, Scholey JM, Vale RD. Length control of the metaphase spindle. Curr Biol. 2005 Nov 22;15(22):1979-88. PMID:16303556 doi:10.1016/j.cub.2005.09.054
- ↑ Maiato H, Khodjakov A, Rieder CL. Drosophila CLASP is required for the incorporation of microtubule subunits into fluxing kinetochore fibres. Nat Cell Biol. 2005 Jan;7(1):42-7. Epub 2004 Dec 12. PMID:15592460 doi:10.1038/ncb1207
- ↑ Laycock JE, Savoian MS, Glover DM. Antagonistic activities of Klp10A and Orbit regulate spindle length, bipolarity and function in vivo. J Cell Sci. 2006 Jun 1;119(Pt 11):2354-61. PMID:16723741 doi:10.1242/jcs.02957
- ↑ De la Mora-Rey T, Guenther BD, Finzel BC. The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jul 1;69(Pt 7):723-9. doi:, 10.1107/S1744309113015182. Epub 2013 Jun 27. PMID:23832196 doi:10.1107/S1744309113015182
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