Structural highlights
Function
[KBTBA_RAT] Substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Required for pseudopod elongation in transformed cells.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Kelch-related protein 1 (Krp1) is up-regulated in oncogene-transformed fibroblasts. The Kelch repeats interact directly with the actin-binding protein Lasp-1 in membrane ruffles at the tips of pseudopodia, where both proteins are necessary for pseudopodial elongation. Herein, we investigate the molecular basis for this interaction. Probing an array of overlapping decapeptides of Rattus norvegicus (Rat) Krp1 with recombinant Lasp-1 revealed two binding sites; one ((317)YDPMENECYLT(327)) precedes the first of five Kelch repeats, and the other ((563)TEVNDIWKYEDD(574)) is in the last of the five Kelch repeats. Mutational analysis established that both binding sites are necessary for Krp1-Lasp-1 interaction in vitro and function in vivo. The crystal structure of the C-terminal domain of rat Krp1 (amino acids 289-606) reveals that both binding sites are brought into close proximity by the formation of a novel six-bladed beta-propeller, where the first blade is not formed by a Kelch repeat.
Novel beta-propeller of the BTB-Kelch protein Krp1 provides a binding site for Lasp-1 that is necessary for pseudopodial extension.,Gray CH, McGarry LC, Spence HJ, Riboldi-Tunnicliffe A, Ozanne BW J Biol Chem. 2009 Oct 30;284(44):30498-507. Epub 2009 Sep 2. PMID:19726686[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Spence HJ, Johnston I, Ewart K, Buchanan SJ, Fitzgerald U, Ozanne BW. Krp1, a novel kelch related protein that is involved in pseudopod elongation in transformed cells. Oncogene. 2000 Mar 2;19(10):1266-76. PMID:10713668 doi:10.1038/sj.onc.1203433
- ↑ Gray CH, McGarry LC, Spence HJ, Riboldi-Tunnicliffe A, Ozanne BW. Novel beta-propeller of the BTB-Kelch protein Krp1 provides a binding site for Lasp-1 that is necessary for pseudopodial extension. J Biol Chem. 2009 Oct 30;284(44):30498-507. Epub 2009 Sep 2. PMID:19726686 doi:10.1074/jbc.M109.023259
