Structural highlights
2wqw is a 2 chain structure with sequence from Listeria monocytogenes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | |
| Related: | 1h6t, 1otm, 2uzx, 1otn, 2uzy, 1m9s, 1oto, 1d0b, 2wqv, 2wqu, 2wqx |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Function
[INLB_LISMO] Mediates the entry of Listeria monocytogenes into cells.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Listeria monocytogenes surface protein InlB mediates bacterial invasion into host cells by activating the human receptor tyrosine kinase Met. So far, it is unknown how InlB or the physiological Met ligand hepatocyte growth factor/scatter factor causes Met dimerization, which is considered a prerequisite for receptor activation. We determined two new structures of InlB, revealing a recurring, antiparallel, dimeric arrangement, in which the two protomers interact through the convex face of the leucine-rich repeat domain. The same contact is found in one structure of the InlB-Met complex. Mutations disrupting the interprotomeric contact of InlB reduced its ability to activate Met and downstream signaling. Conversely, stabilization of this crystal contact by two intermolecular disulfide bonds generates a constitutively dimeric InlB variant with exceptionally high signaling activity, which can stimulate cell motility and cell division. These data demonstrate that the signaling-competent InlB-Met complex assembles with 2:2 stoichiometry around a back-to-back InlB dimer, enabling the direct contact between the stalk region of two Met molecules.
Ligand-mediated dimerization of the Met receptor tyrosine kinase by the bacterial invasion protein InlB.,Ferraris DM, Gherardi E, Di Y, Heinz DW, Niemann HH J Mol Biol. 2010 Jan 22;395(3):522-32. Epub 2009 Nov 6. PMID:19900460[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ferraris DM, Gherardi E, Di Y, Heinz DW, Niemann HH. Ligand-mediated dimerization of the Met receptor tyrosine kinase by the bacterial invasion protein InlB. J Mol Biol. 2010 Jan 22;395(3):522-32. Epub 2009 Nov 6. PMID:19900460 doi:10.1016/j.jmb.2009.10.074
