2irv
From Proteopedia
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| , resolution 2.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Gene: | glpG (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of GlpG, a rhomboid intramembrane serine protease
Overview
Intramembrane proteases catalyze peptide bond cleavage of integral membrane protein substrates. This activity is crucial for many biological and pathological processes. Rhomboids are evolutionarily widespread intramembrane serine proteases. Here, we present the 2.3-A-resolution crystal structure of a rhomboid from Escherichia coli. The enzyme has six transmembrane helices, five of which surround a short TM4, which starts deep within the membrane at the catalytic serine residue. Thus, the catalytic serine is in an externally exposed cavity, which provides a hydrophilic environment for proteolysis. Our results reveal a mechanism to enable water-dependent catalysis at the depth of the hydrophobic milieu of the membrane and suggest how substrates gain access to the sequestered rhomboid active site.
About this Structure
2IRV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural basis for intramembrane proteolysis by rhomboid serine proteases., Ben-Shem A, Fass D, Bibi E, Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):462-6. Epub 2006 Dec 26. PMID:17190827
Page seeded by OCA on Thu Mar 20 17:32:31 2008
Categories: Escherichia coli | Single protein | Ben-Shem, A. | Bibi, E. | Fass, D. | LDA | LMT | PGV | PO4 | Cavity | Membrane protein | Ser-his dyad
