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From Proteopedia
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BARLEY ALDOSE REDUCTASE 1 COMPLEX WITH BUTANOL
Cytokinin deshydrogenase(Wikipedia), also called CKX, is an enzyme which degrades cytokinin hormones in plants and which is encoded by the gene ZmCKX1. The one is located in Zea Maize on the chromosome 3 (NCBI Gene ID: 542585) and belongs to a multigene family called Vanillyl-Alcohol Oxidase (VAO) flavoprotein family. The gene is more particularly expressed in the kernel of maize, mainly in the embryo since it may protect the embryo from too much cytokinin and permit the correct development. [1]
Cytokine deshydrogenase are extracellular and monomeric proteins with a molecular weight of 63kDa.[2][2] For protein production purposes, ZmCKO1 precursor protein was truncated by deletion of 18 N-terminal amino acids to produce the expected mature enzyme. [8] The Enzyme Classification number of CKX is EC 1.5.99.12 and this indicates that the enzyme is an oxydo reductase which acts on the CH-NH group of the donor. Consequently the reaction of CKX with its cytokinin substrate is a transfer of two electrons from the cytokinin to an acceptor which is in the case of CKX the Flavine Adenine Nucleotide (FAD) cofactor. [1]
References
- ↑ Frebortova J, Novak O, Frebort I, Jorda R. Degradation of cytokinins by maize cytokinin dehydrogenase is mediated by free radicals generated by enzymatic oxidation of natural benzoxazinones. Plant J. 2010 Feb 1;61(3):467-81. doi: 10.1111/j.1365-313X.2009.04071.x. Epub, 2009 Nov 14. PMID:19912568 doi:http://dx.doi.org/10.1111/j.1365-313X.2009.04071.x
- ↑ 2.0 2.1 Kopecny D, Briozzo P, Popelkova H, Sebela M, Koncitikova R, Spichal L, Nisler J, Madzak C, Frebort I, Laloue M, Houba-Herin N. Phenyl- and benzylurea cytokinins as competitive inhibitors of cytokinin oxidase/dehydrogenase: a structural study. Biochimie. 2010 Aug;92(8):1052-62. Epub 2010 May 15. PMID:20478354 doi:10.1016/j.biochi.2010.05.006
