1gzm

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STRUCTURE OF BOVINE RHODOPSIN IN A TRIGONAL CRYSTAL FORM

Overview

We have determined the structure of bovine rhodopsin at 2.65 A resolution, using untwinned native crystals in the space group P3(1), by molecular, replacement from the 2.8 A model (1F88) solved in space group P4(1). The, new structure reveals mechanistically important details unresolved, previously, which are considered in the membrane context by docking the, structure into a cryo-electron microscopy map of 2D crystals. Kinks in the, transmembrane helices facilitate inter-helical polar interactions. Ordered, water molecules extend the hydrogen bonding networks, linking Trp265 in, the retinal binding pocket to the NPxxY motif near the cytoplasmic, boundary, and the Glu113 counterion for the protonated Schiff base to the, extracellular surface. Glu113 forms a complex with a water molecule, ... [(full description)]

About this Structure

1GZM is a [Single protein] structure of sequence from [Bos taurus] with ZN, ACE, RET, PLM, PEF, LDA and C8E as [ligands]. Full crystallographic information is available from [OCA].

Reference

Structure of bovine rhodopsin in a trigonal crystal form., Li J, Edwards PC, Burghammer M, Villa C, Schertler GF, J Mol Biol. 2004 Nov 5;343(5):1409-38. PMID:15491621

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