2pnh
From Proteopedia
| |||||||
| , resolution 2.250Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | priB (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Escherichia coli PriB E39A variant
Overview
Collapsed DNA replication forks must be reactivated through origin-independent reloading of the replication machinery (replisome) to ensure complete duplication of cellular genomes. In E. coli, the PriA-dependent pathway is the major replication restart mechanism and requires primosome proteins PriA, PriB, and DnaT for replisome reloading. However, the molecular mechanisms that regulate origin-independent replisome loading are not fully understood. Here, we demonstrate that assembly of primosome protein complexes represents a key regulatory mechanism, as inherently weak PriA-PriB and PriB-DnaT interactions are strongly stimulated by single-stranded DNA. Furthermore, the binding site on PriB for single-stranded DNA partially overlaps the binding sites for PriA and DnaT, suggesting a dynamic primosome assembly process in which single-stranded DNA is handed off from one primosome protein to another as a repaired replication fork is reactivated. This model helps explain how origin-independent initiation of DNA replication is restricted to repaired replication forks, preventing overreplication of the genome.
About this Structure
2PNH is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A hand-off mechanism for primosome assembly in replication restart., Lopper M, Boonsombat R, Sandler SJ, Keck JL, Mol Cell. 2007 Jun 22;26(6):781-93. PMID:17588514
Page seeded by OCA on Thu Mar 20 18:15:11 2008
