2qlv

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spinqualitylabelsall models PDB ID 2qlv Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
, resolution 2.600Å
Gene:	SNF1, CAT1, CCR1, GLC2, PAS14 (Saccharomyces cerevisiae), SIP2, SPM2 (Saccharomyces cerevisiae), SNF4, CAT3 (Saccharomyces cerevisiae)
Activity:	Non-specific serine/threonine protein kinase, with EC number 2.7.11.1
Coordinates:	save as pdb, mmCIF, xml

Crystal structure of the heterotrimer core of the S. cerevisiae AMPK homolog SNF1

Overview

AMP-activated protein kinase (AMPK) is a central regulator of energy homeostasis in mammals and is an attractive target for drug discovery against diabetes, obesity and other diseases. The AMPK homologue in Saccharomyces cerevisiae, known as SNF1, is essential for responses to glucose starvation as well as for other cellular processes, although SNF1 seems to be activated by a ligand other than AMP. Here we report the crystal structure at 2.6 A resolution of the heterotrimer core of SNF1. The ligand-binding site in the gamma-subunit (Snf4) has clear structural differences from that of the Schizosaccharomyces pombe enzyme, although our crystallographic data indicate that AMP can also bind to Snf4. The glycogen-binding domain in the beta-subunit (Sip2) interacts with Snf4 in the heterotrimer but should still be able to bind carbohydrates. Our structure is supported by a large body of biochemical and genetic data on this complex. Most significantly, the structure reveals that part of the regulatory sequence in the alpha-subunit (Snf1) is sequestered by Snf4, demonstrating a direct interaction between the alpha- and gamma-subunits and indicating that our structure may represent the heterotrimer core of SNF1 in its activated state.

About this Structure

2QLV is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Crystal structure of the heterotrimer core of Saccharomyces cerevisiae AMPK homologue SNF1., Amodeo GA, Rudolph MJ, Tong L, Nature. 2007 Sep 27;449(7161):492-5. Epub 2007 Sep 12. PMID:17851534

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