Elongation factor

From Proteopedia

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spinqualitylabelsall models Structure of EF-Tu (grey and pink) with EF-Ts (green and yellow) (PDB entry 1efu) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Elongation factors (EF) facilitate translational elongation during the formation of peptide bonds in the ribosome. EF-selB is selenocysteine-specific EF. EF-Tu or EF 1-α (elongation factor thermo unstable) is a prokaryotic EF. EF-Tu contributes to translational accuracy. It catalyzes the addition of aminoacyl tRNA. EF-Ts or EF 1-β (elongation factor thermo stable) catalyzes the release of GDP from EF-Tu. EF-G translocates the peptidyl tRNA from the A site to the P site while moving the mRNA through the ribosome. EF-SII helps RNA polymerase II to bypass blocks to elongation. EF-ELL2 enhances polyadenylation and exon skipping with the gene encoding the immunoglobulin heavy-chain complex. EF-GreA or GreB are cleavage factors allowing the resumption of elongation. EF-NusA recruits translesion DNA polymerases to gaps encountered during translation. EF-P alters the ribosome affinity to aminoacyl-tRNA. EF-1 gamma acts during the delivery of aminoacyl tRNA to the ribosome. EF-2 promotes the translocation of the nascent protein chain from the A site to the P site on the ribosome. EF-3 is unique EF in fungi hence it provides an anti-fungal drug target. EF Spt4, Spt5, Spt6 are conserved among eukaryotes. They modulate the chromatin structure. EF-CA150 is believed to play a role in coupling transcription and splicing. The elongin B and C complex is involved in the proteasomal degredation of target proteins. For EF-3 see HEAT Repeat For EF-SelB see SelB.

3D structures of elongation factor

Updated on 29-March-2015 {{#tree:id=OrganizedByTopic|openlevels=0|

• EF-Tu

• • 1efm, 1efc, 1dg1, 2fx3, 1etu – EcEF – Escherichia coli
    
  • 1qzd – EcEF – Cryo EM
    
  • 3u2q – EcEF + drug
    
  • 1mj1 – EcEF + Phe-tRNA + S12 + S13 + L11 – Cryo EM
    
  • 3ep2 - EcEF + tRNA + S12 + L11 + 16SRRNA + 23SRRNA – Cryo EM
    
  • 1ttt, 1ob5 – TaEF + Phe-tRNA + GDPNP - Thermus aquaticus
    
  • 1b23 - TaEF + Cys-tRNA
    
  • 1ls2 – yEF + Phe-tRNA – yeast – Cryo EM
    
  • 3agj – EF + plethora protein – Aeropyrum pernix
    
  • 4h9g, 4lbv, 4lbw, 4lby, 4lbz, 4lc0 – TtEF – Thermus thermophilus
    
  • 4j0q – EF – Pseudomonas putida
    

• EF-Tu complex with antibiotics

• • 1ob2 - yEF + Phe-tRNA + antibiotic
    
  • 4abr - TtEF + antibiotic in 30S ribosome
    
  • 1ha3 - TtEF + antibiotic + GDP
    
  • 2c77, 2c78 - TtEF + antibiotic + GTP analog
    
  • 3fic, 3fic – TtEF + antibiotic in 70S ribosome – Cryo EM
    
  • 2bvn - EcEF + GDP
    NP + antibiotic
    
  • 1d8t, 2hcj, 2hdn, 3u6b, 3u6k, 4g5g – EcEF + antibiotic

• EF-Tu complex with nucleotide

• • 1efu – EcEF + GDP
    
  • 1exm – TtEF + GTP analog
    
  • 1eft – TaEF + GNP – Thermus aquaticus
    
  • 1tui – TaEF + GDP
    
  • 1d2e – bEF + GDP – bovine
    
  • 1jny, 1skq – SsEF + GDP – Sulfolobus solfataricus

• EF-Tu with EF-Ts

• • 1efu – EcEF + EcEF-Ts
    
  • 1aip - TtEF + TtEF-Ts - Thermus thermophilus
    
  • 1xb2 – bEF + EF-Ts - bovine
    
  • 1f60 – yEF + EF-Ts C terminal
    
  • 1g7c - yEF + EF-Ts C terminal + GDPNP
    
  • 1ije, 1ijf - yEF + EF-Ts C terminal + GDP
    
  • 2b7b - yEF + EF-Ts C terminal (mutant) + GDP
    
  • 2b7c - yEF + EF-Ts C terminal (mutant)

• EF-Tu in the ribosome

• • 3eq3, 3eq4 – EcEF in 80S ribosome – Cryo EM
    
  • 3izv, 3izw, 3fih - EcEF in 30S ribosome – Cryo EM
    
  • 1zc8, 3dwu – TtEF in 70S ribosome – Cryo EM
    
  • 2wrn, 2wrq, 2xqd, 2y0u, 2y0w, 2y0y, 2y10, 2y12, 2y14, 2y16, 2y18 - TtEF in 70S ribosome

• EF-SII

• • 1tfi – hEF – human – NMR
    
  • 3ndq - hEF domain II
    
  • 1enw – yEF domain II – NMR
    
  • 1eo0 - yEF domain I – NMR
    
  • 2xex – SaEF – Staphylococcus aureus
    
  • 1pqv, 1y1v – yEF + RNA polymerase II
    
  • 1y1y, 3gtm – yEF + RNA polymerase II + RNA
    
  • 1wjt – mEF N terminal – mouse – NMR
    
  • 2m1h – EF PWWP domain – Trypanosoma brucei – NMR
    

• EF-ELL2

• • 2e5n – hEF N2 domain - NMR
    

• EF-G

• • 1efg, 1elo, 1ktv, 1wdt, 2dy1 – TtEF
    
  • 1pn6, 3izp – TtEF – Cryo EM
    
  • 4b8f, 4b8h – TtEF in 70S ribosome
    
  • 4juw, 4kbt, 4kbv, 4kcy, 4kd0, 4kd8, 4kda, 4kdg, 4kdj, 4btc – TtEF in 30S ribosome
    
  • 4kiy, 4kj0, 4kj2, 4kj4, 4kj6, 4kj8, 4kja, 4kjc – EcEF in 30S ribosome
    
  • 3j5n – EcEF in 30S ribosome – Cryo-EM
    
  • 3j5x – EcEF in 30S ribosome + antibiotics – Cryo-EM
    
  • 1fnm, 2bm0, 2bm1 – TtEF (mutant)
    
  • 2bcw – TtEF + L11 + L7/L12
    
  • 2xex – SaEF
    
  • 3zz0, 3zzt, 3zzu – SaEF (mutant)
    
  • 2bv3 - TtEF (mutant) + GTP analog
    
  • 2j7k - TtEF (mutant) + GDP analog
    
  • 2om7, 2wri, 2wrk – TtEF in 70S ribosome – Cryo EM
    
  • 2xsy, 2xuy - TtEF in 70S ribosome
    
  • 1dar, 2efg – TtEF + GDP
    
  • 1jqm, 1jqs – EcEF + L11 – Cryo EM
    
  • 1zn0, 2rdo, 3j0e, 3j18 – EcEF in 30S ribosome – Cryo-EM
    
  • 4fn5 – PaEF - Pseudomonas aeruginosa
    

• EF-GreA/GreB

• • 2pn0 – EF – Nitrosomonas europaea
    
  • 2p4v – EcEF-GreB
    
  • 1grj – EcEF-GreA
    

• EF-NusA

• • 1wcl, 1wcn – EcEF C terminal – NMR
    
  • 2kwp - EcEF N terminal – NMR
    
  • 2jzb – EcEF + RNA polymerase subunit α - NMR
    

• EF-P

• • 1ueb – TtEF
    
  • 3huw, 3huy – TtEF in 70S ribosome
    
  • 3oyy – PaEF
    
  • 3tre – EF – Coxiella burnetii
    
  • 3a5z – EcEF + lysyl-tRNA synthetase

• EF-Ts

• • 1tfe – TtEF
    
  • 1b64 – hEF guanine exchange factor domain – NMR
    
  • 2cp9 – UBA domain – NMR
    
  • 1gh8 – EF – Methanobacterium thermoautotrophicum – NMR
    
  • 2yy3 – EF – Pyrococcus horikoshii
    
  • 2uz8 – hEF (mutant)

• EF-1G

• • 1nhy – yEF N terminal
  • 1pbu – hEF C terminal (mutant) - NMR
    

• EF-2

• • 1n0v – yEF
    
  • 1u2r – yEF + GDP
    
  • 1n0u, 2e1r, 2npf – yEF + antifungal drug
    
  • 1zm2, 1zm3, 1zm4, 1zm9, 3b78, 3b82, 3b8h, 2zit – yEF + exotoxin
    
  • 1s1h - yEF + antifungal drug in 40S ribosome – Cryo EM
    
  • 1s1h - hEF in 40S ribosome – Cryo EM
    
  • 2p8w, 2p8x, 3dny - yEF in 80S ribosome – Cryo EM
    
  • 2p8y, 2p8z - yEF+ antifungal drug in 80S ribosome – Cryo EM
    

• EF-3

• • 2ix3 – yEF
    
  • 2iwh – yEF + ADPNP
    
  • 2iw3 – yEF + ADP
    
  • 2ix8 – yEF in 80S ribosome – Cryo EM
    

• EF-SelB

• • 1lva – MtEF C terminal – Moorella thermoacetica
    
  • 2v9v – MtEF winged helix domain
    
  • 1wsu, 2uwm – MtEF + RNA
    
  • 2ply – MtEF (mutant) + RNA
    
  • 1wb2, 4aca – MmEF – Methanococcus maripaludis
    
  • 1wb3, 4acb – MmEF + GTP analog
    
  • 4ac9, 1wb1 – MmEF + GDP
    
  • 2pjp – EcEF + RNA

• EF-Spt5

• • 2do3, 2e6z, 2e70 – hEF KOW motif – NMR
    
  • 2exu – yEF
    
  • 3h7h – hEF + hEF-Spt4

• EF-Spt6

• • 3gxw, 3gxx, 3pjp – EF SH2 domain – Candida glabrata
    
  • 3psf, 3psi – yEF core domain
    
  • 3psj, 3psk – yEF SH2 domain
    
  • 2l3t - yEF SH2 domain]] - NMR
    
  • 3oak – yEF + transcription factor IWS1

• EF-CA150

• • 2dod, 2doe, 2dof, 2e71, 2kiq, 2kis – hEF FF domain – NMR
    
  • 3hfh – hEF FF domain
    
  • 2ysi– mEF WW domain - NMR
    

• Elongin BC complex

• • 1lqb, 1lm8 – hEloBC + von-Hippel Lindau disease tumor suppressor + hypoxia inducible factor 1 α
    
  • 3zrc, 3ztc, 3ztd, 3zun - hEloBC + von-Hippel Lindau disease tumor suppressor + inhibitor
    
  • 3zrf - hEloBC + von-Hippel Lindau disease tumor suppressor
    
  • 2c9w, 2izv, [[2jz3 – hEloBC + suppressor of cytokine signaling
    
  • 3dcg – hEloBC + virion infectivity factor
    
  • 2xai - hEloBC + ankyrin rep
    

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