2v0r
From Proteopedia
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CRYSTAL STRUCTURE OF A HAIRPIN EXCHANGE VARIANT (LTX) OF THE TARGETING LINE-1 RETROTRANSPOSON ENDONUCLEASE
Overview
The human LINE-1 endonuclease (L1-EN) is the targeting endonuclease encoded by the human LINE-1 (L1) retrotransposon. L1-EN guides the genomic integration of new L1 and Alu elements that presently account for approximately 28% of the human genome. L1-EN bears considerable technological interest, because its target selectivity may ultimately be engineered to allow the site-specific integration of DNA into defined genomic locations. Based on the crystal structure, we generated L1-EN mutants to analyze and manipulate DNA target site recognition. Crystal structures and their dynamic and functional analysis show entire loop grafts to be feasible, resulting in altered specificity, while individual point mutations do not change the nicking pattern of L1-EN. Structural parameters of the DNA target seem more important for recognition than the nucleotide sequence, and nicking profiles on DNA oligonucleotides in vitro are less well defined than the respective integration site consensus in vivo. This suggests that additional factors other than the DNA nicking specificity of L1-EN contribute to the targeted integration of non-LTR retrotransposons.
About this Structure
2V0R is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Determinants for DNA target structure selectivity of the human LINE-1 retrotransposon endonuclease., Repanas K, Zingler N, Layer LE, Schumann GG, Perrakis A, Weichenrieder O, Nucleic Acids Res. 2007;35(14):4914-26. Epub 2007 Jul 10. PMID:17626046
Page seeded by OCA on Thu Mar 20 18:42:04 2008
