User:Wayne Decatur/Gal 4

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Gal4p is a transcriptional activator in Saccharomyces cerevisiae that regulates the expression of genes to coordinate the response to the carbon source galactose. Structures

spinqualitylabelsall models The N-terminal amino acids of Gal4p bound to DNA. Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

DNA Recognition by Gal4p

Gal4p is a transcriptional activator in Saccharomyces cerevisiae that regulates the expression of genes to coordinate the response to the carbon source galactose. Gal4p is an 881-amino-acid protein with a Zn cluster-type DNA-binding domain, a linker domain, a dimerization domain, and two acidic activation domains. The structure of The protein binds as a dimer to a symmetrical 17-base-pair sequence. Specifically, the consensus Gal4-binding site is a 17-mer of sequence conforming to the motif below.
        5'-CGGNNNNNNNNNNNCCG-3'
           |||||||||||||||||
        3'-CGGNNNNNNNNNNNGGC-5'

Pioneering studies on Gal4p from the Ptashne laboratory shaped our understanding of transcriptional activation in eukaryotes and Gal4p derivatives, along with the congnate promoters, have been used as tools in yeast for many years.

Reference

• Marmorstein R, Carey M, Ptashne M, Harrison SC. DNA recognition by GAL4: structure of a protein-DNA complex. Nature. 1992 Apr 2;356(6368):408-14. PMID:1557122 doi:http://dx.doi.org/10.1038/356408a0