3b4c

From Proteopedia

Revision as of 16:56, 20 March 2008 by OCA (Talk | contribs)
Jump to: navigation, search
Image:3b4c.gif


PDB ID 3b4c

Drag the structure with the mouse to rotate
, resolution 3.0Å
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



T. tengcongensis glmS ribozyme bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage


Overview

The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.

About this Structure

3B4C is a Protein complex structure of sequences from Thermoanaerobacter tengcongensis. Full crystallographic information is available from OCA.

Reference

Essential role of an active-site guanine in glmS ribozyme catalysis., Klein DJ, Been MD, Ferre-D'Amare AR, J Am Chem Soc. 2007 Dec 5;129(48):14858-9. Epub 2007 Nov 9. PMID:17990888

Page seeded by OCA on Thu Mar 20 18:55:59 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3b4c"
Personal tools