The [NiFe] hydrogenase contains a NiFe(CO)(CN)2 in its reaction center. HypA, HypB, HypC, HypD, HypE and HypF are required for the maturation of [NiFe] hydrogenase. HypA and HypB are involved in the insertion of Ni atom. HypE and HypF catalyze the biosynthesis of the CN ligand. HypC and HypD deliver the Fe and CO to the hydrogenase active center. For more details of HypA see Molecular Playground/HypA.
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Structural highlights