Structural highlights
5chb is a 3 chain structure. This structure supersedes the now removed PDB entry 4zco. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Publication Abstract from PubMed
We have engineered a metal-binding site into the novel artificial beta-propeller protein Pizza. This new Pizza variant carries two nearly identical domains per polypeptide chain, and forms a trimer with three-fold symmetry. The designed single metal ion binding site lies on the symmetry axis, bonding the trimer together. Two copies of the trimer associate in the presence of cadmium chloride in solution, and very high-resolution X-ray crystallographic analysis reveals a nanocrystal of cadmium chloride, sandwiched between two trimers of the protein. This nanocrystal, containing seven cadmium ions lying in a plane and twelve interspersed chloride ions, is the smallest reported to date. Our results indicate the feasibility of using rationally designed symmetrical proteins to biomineralize nanocrystals with useful properties.
Biomineralization of a Cadmium Chloride Nanocrystal by a Designed Symmetrical Protein.,Voet AR, Noguchi H, Addy C, Zhang KY, Tame JR Angew Chem Int Ed Engl. 2015 Jul 1. doi: 10.1002/anie.201503575. PMID:26136355[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Voet AR, Noguchi H, Addy C, Zhang KY, Tame JR. Biomineralization of a Cadmium Chloride Nanocrystal by a Designed Symmetrical Protein. Angew Chem Int Ed Engl. 2015 Jul 1. doi: 10.1002/anie.201503575. PMID:26136355 doi:http://dx.doi.org/10.1002/anie.201503575
