1nrg

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models PDB ID 1nrg Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
, resolution 1.95Å
Ligands:	, , and
Gene:	Human (Homo sapiens)
Coordinates:	save as pdb, mmCIF, xml

Structure and Properties of Recombinant Human Pyridoxine-5'-Phosphate Oxidase

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Pyridoxine 5'-phosphate oxidase catalyzes the terminal step in the synthesis of pyridoxal 5'-phosphate. The cDNA for the human enzyme has been cloned and expressed in Escherichia coli. The purified human enzyme is a homodimer that exhibits a low catalytic rate constant of approximately 0.2 sec(-1) and K(m) values in the low micromolar range for both pyridoxine 5'phosphate and pyridoxamine 5'-phosphate. Pyridoxal 5'-phosphate is an effective product inhibitor. The three-dimensional fold of the human enzyme is very similar to those of the E. coli and yeast enzymes. The human and E. coli enzymes share 39% sequence identity, but the binding sites for the tightly bound FMN and substrate are highly conserved. As observed with the E. coli enzyme, the human enzyme binds one molecule of pyridoxal 5'-phosphate tightly on each subunit.

Disease

Known disease associated with this structure: Pyridoxamine 5 -phosphate oxidase deficiency OMIM:[603287]

About this Structure

1NRG is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase., Musayev FN, Di Salvo ML, Ko TP, Schirch V, Safo MK, Protein Sci. 2003 Jul;12(7):1455-63. PMID:12824491

Page seeded by OCA on Sun Mar 23 12:56:46 2008