Structural highlights
Function
[NXS1_NAJOX] Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Binds to the muscular nicotinic acetylcholine receptor.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The spatial structure of neurotoxin II from the venom of the central Asian cobra Naja naja oxiana was determined by two-dimensional 1H-NMR techniques and computational analysis. Nearly complete proton resonance assignments for 61 amino acid residues have been made using two-dimensional (2D) homonuclear total correlated spectroscopy, 2D homonuclear double-quantum-filtered correlated spectroscopy and 2D homonuclear NOE spectroscopy (NOESY) experiments. The cross-peak volumes in NOESY spectra spin-spin coupling constants of vicinal protons NH-C alpha H and C alpha H-C beta H and the observation of slow deuterium exchange of amide protons were used to define local structure and a set of constraints for distance geometry program DIANA. The average root-mean-square deviations are 53 pm for backbone heavy atoms and 118 pm for all heavy atoms of 19 final neurotoxin II conformations. The spatial structure is characterized by a short double-stranded (residues 1-5 and 13-17) and a triple-stranded (residues 22-30, 33-41 and 50-54) antiparallel beta-sheets.
Two-dimensional 1H-NMR study of the spatial structure of neurotoxin II from Naja naja oxiana.,Golovanov AP, Lomize AL, Arseniev AS, Utkin YN, Tsetlin VI Eur J Biochem. 1993 May 1;213(3):1213-23. PMID:8504813[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Golovanov AP, Lomize AL, Arseniev AS, Utkin YN, Tsetlin VI. Two-dimensional 1H-NMR study of the spatial structure of neurotoxin II from Naja naja oxiana. Eur J Biochem. 1993 May 1;213(3):1213-23. PMID:8504813
