Structural highlights
Publication Abstract from PubMed
The crystal and molecular structure of the first DNA duplex containing the carcinogenic lesion O6MeG has been determined to a resolution of 1.9 A and refined to an R factor of 19%. (d[CGC-(O6Me)GCG])2 crystallizes in the left-handed Z DNA form and has crystal parameters and conformational features similar to those of the parent sequence [d(CG)3]2. The methyl groups on O6 of G4 and G10 have C5-C6-O6-O6Me torsion angles of 73 degrees and 56 degrees, respectively, and protrude onto the major groove surface. The base-pairing conformation for the methylated G.C base pairs is of the Watson-Crick type as opposed to a wobble-type conformation that had been proposed in a B DNA fragment. As in other Z DNA structures, a spine of hydration is seen in the minor groove.
Crystal and molecular structure of a DNA duplex containing the carcinogenic lesion O6-methylguanine.,Ginell SL, Kuzmich S, Jones RA, Berman HM Biochemistry. 1990 Nov 20;29(46):10461-5. PMID:2271657[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ginell SL, Kuzmich S, Jones RA, Berman HM. Crystal and molecular structure of a DNA duplex containing the carcinogenic lesion O6-methylguanine. Biochemistry. 1990 Nov 20;29(46):10461-5. PMID:2271657
