1rzn

Structural highlights

Function

[RECU_BACSU] Has at least 2 separable functions; Holliday junction resolution with generation of monomeric chromosomes, and modulation of RecA activity. Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair, homologous recombination and chromosome segregation. Partially inhibits the hydrolysis of dATP or rATP by RecA. Holliday junction resolution is stimulated by RuvB.^{[1] [2] [3]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

1. ↑ Ayora S, Carrasco B, Doncel-Perez E, Lurz R, Alonso JC. Bacillus subtilis RecU protein cleaves Holliday junctions and anneals single-stranded DNA. Proc Natl Acad Sci U S A. 2004 Jan 13;101(2):452-7. Epub 2003 Dec 30. PMID:14701911 doi:http://dx.doi.org/10.1073/pnas.2533829100
2. ↑ Sanchez H, Kidane D, Reed P, Curtis FA, Cozar MC, Graumann PL, Sharples GJ, Alonso JC. The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis. Genetics. 2005 Nov;171(3):873-83. Epub 2005 Jul 14. PMID:16020779 doi:http://dx.doi.org/genetics.105.045906
3. ↑ McGregor N, Ayora S, Sedelnikova S, Carrasco B, Alonso JC, Thaw P, Rafferty J. The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence-specific cleavage. Structure. 2005 Sep;13(9):1341-51. PMID:16154091 doi:10.1016/j.str.2005.05.011