Structural highlights
Function
[PROS_DROME] Required for proper neuronal differentiation of most or all neurons and their precursors in central and peripheral nervous systems, axonal outgrowth and pathfinding. Not required for the specification of neuronal identity. May regulate transcription by binding to DNA.[1] [2] [3] [4]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The crystal structure of a complex between the novel homeodomain of the neural transcription factor Prospero and DNA shows that the invariant residues Lys1290, Asn1294, and Asp1297 make specific contacts with the noncanonical DNA binding site. The overall structure includes the homeodomain and the adjacent Prospero domain and confirms that they act as a single structural unit, a Homeo-Prospero domain. The Prospero domain facilitates the proper alignment of the protein on the DNA. Knowledge of the structure reconciles two different DNA sequences that have been proposed as transcriptional targets for Prospero. As in the apo structure, the C terminus of the Prospero domain shields a short helix within the homeodomain that includes a nuclear export signal (NES). The structural results suggest that exposure of the NES is not coupled directly to DNA binding. We propose a DNA recognition mechanism specific to Prospero-type homeodomains in developing cells.
Structural basis of Prospero-DNA interaction: implications for transcription regulation in developing cells.,Yousef MS, Matthews BW Structure. 2005 Apr;13(4):601-7. PMID:15837198[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vaessin H, Grell E, Wolff E, Bier E, Jan LY, Jan YN. prospero is expressed in neuronal precursors and encodes a nuclear protein that is involved in the control of axonal outgrowth in Drosophila. Cell. 1991 Nov 29;67(5):941-53. PMID:1720353
- ↑ Chu-Lagraff Q, Wright DM, McNeil LK, Doe CQ. The prospero gene encodes a divergent homeodomain protein that controls neuronal identity in Drosophila. Development. 1991;Suppl 2:79-85. PMID:1842358
- ↑ Matsuzaki F, Koizumi K, Hama C, Yoshioka T, Nabeshima Y. Cloning of the Drosophila prospero gene and its expression in ganglion mother cells. Biochem Biophys Res Commun. 1992 Feb 14;182(3):1326-32. PMID:1540176
- ↑ Xu C, Kauffmann RC, Zhang J, Kladny S, Carthew RW. Overlapping activators and repressors delimit transcriptional response to receptor tyrosine kinase signals in the Drosophila eye. Cell. 2000 Sep 29;103(1):87-97. PMID:11051550
- ↑ Yousef MS, Matthews BW. Structural basis of Prospero-DNA interaction: implications for transcription regulation in developing cells. Structure. 2005 Apr;13(4):601-7. PMID:15837198 doi:10.1016/j.str.2005.01.023
