1uwt
From Proteopedia
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STRUCTURE OF BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS IN COMPLEX WITH D-GALACTOHYDROXIMO-1,5-LACTAM
Overview
Transition-state mimicry is increasingly important both to understand, enzyme mechanism and to direct the synthesis of putative therapeutic, agents. X-ray crystallography is able to provide vital information on the, interactions between an enzyme and the potential inhibitor. Here we report, the structures, at approximately 2 A resolution, of a family GH1, beta-glycosidase from the hyperthermophilic archaeon Sulfolobus, solfataricus, in complex with both covalently (derived from, 2-fluoro-glycosides) and noncovalently (hydroximolactam) bound inhibitors., The enzyme has broad specificity, accommodating both gluco- and, galacto-configured substrates, and the crystallographic data demonstrate, that the only difference in the way these ligands bind lies in the, interactions between Gln18, ... [(full description)]
About this Structure
1UWT is a [Single protein] structure of sequence from [Sulfolobus solfataricus] with ACT and GTL as [ligands]. Active as [[1]], with EC number [3.2.1.23]. Full crystallographic information is available from [OCA].
Reference
Structural studies of the beta-glycosidase from Sulfolobus solfataricus in complex with covalently and noncovalently bound inhibitors., Gloster TM, Roberts S, Ducros VM, Perugino G, Rossi M, Hoos R, Moracci M, Vasella A, Davies GJ, Biochemistry. 2004 May 25;43(20):6101-9. PMID:15147194
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