CAS9</scene>' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />
The CRISPR-associated endonuclease Cas9 has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences.
Articles in Proteopedia concerning Cas9 include:
3D structures of Cas9
Streptococcus pyogenes Cas9
- 4un3, 4un4, and 4un5 - S. pyogenes Cas9 bound to sgRNA and target DNA
- 4oo8 - S. pyogenes Cas9 bound to sgRNA and target DNA
- 4cmp
- 4cmq - Mn2+-bound S. pyogenes Cas9
Actinomyces naeslundii Cas9
- 4oge
- 4ogc - Mn2+-bound A.s naeslundii Cas9
See Also
HNH endonuclease
References
The CRISPR-associated endonuclease Cas9 has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences.
Articles in Proteopedia concerning Cas9 include:
3D structures of Cas9
Streptococcus pyogenes Cas9
- 4un3, 4un4, and 4un5 - S. pyogenes Cas9 bound to sgRNA and target DNA
- 4oo8 - S. pyogenes Cas9 bound to sgRNA and target DNA
- 4cmp
- 4cmq - Mn2+-bound S. pyogenes Cas9
Actinomyces naeslundii Cas9
- 4oge
- 4ogc - Mn2+-bound A.s naeslundii Cas9
See Also
HNH endonuclease
References
