Sandbox viralpackagingmotors

From Proteopedia

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Viral Packaging

One of the most important stage in the life cycle of all viruses is the encapsidation (packaging) of the viral genome. Many virus package their genome into preformed capsids using packaging motors powered by the hydrolysis of ATP. The hexameric ATPase P4 of dsRNA bacteriophage phi 12, located at the vertices of the icosahedral capsid is such a packaging motor. ^[1] P4 protein is a 35-kDa ssRNA packaging ATPase Viruses like tailed dsDNA bacteriophages among others package their genome into empty capsids via a portal vertex complex. The RNA packaging motor is powered by the hydrolysis of ATP.

Function

The RNA packaging motor is a straightforward machinery that consists of a portal protein (P4) hexamer. It is the P4 protein that provides energy for RNA translocation.

Energetics

Structural highlights

PDB 1W44 Atomic Snapshots of an RNA Packaging motor. Arg residue highlighted in magenta

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References

1. ↑ Mancini EJ, Kainov DE, Grimes JM, Tuma R, Bamford DH, Stuart DI. Atomic snapshots of an RNA packaging motor reveal conformational changes linking ATP hydrolysis to RNA translocation. Cell. 2004 Sep 17;118(6):743-55. PMID:15369673 doi:10.1016/j.cell.2004.09.007