Structural highlights
2q9o is a 2 chain structure with sequence from Melao. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , , , , , |
| NonStd Res: | |
| Related: | 1gw0, 2ih8, 2ih9 |
| Gene: | LAC1 (MELAO) |
| Activity: | Laccase, with EC number 1.10.3.2 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum |
Function
[LAC1_MELAO] Lignin degradation and detoxification of lignin-derived products (Probable).[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
We have solved a crystal structure from Melanocarpus albomyces laccase expressed in the filamentous fungus Trichoderma reesei (rMaL) at 1.3A resolution by using synchrotron radiation at 100K. At the moment, this is the highest resolution that has been attained for any multicopper oxidase. The present structure confirmed our earlier proposal regarding the dynamic behaviour of the copper cluster. Thermal ellipsoids of copper atoms indicated movements of trinuclear site coppers. The direction of the type-3 copper motion was perpendicular to the type-2 copper. In addition, the structure at 1.3A resolution allowed us to describe important solvent cavities of the enzyme and the structure is also compared with other known multicopper oxidases. T2 and T3 solvent cavities, and a putative SDS-gate, formed by Ser142, Ser510 and the C-terminal Asp556 of rMaL, are described. We also observed a 2-oxohistidine, an oxidized histidine, possibly caused by a metal-catalysed oxidation by the trinuclear site coppers. To our knowledge, this is the first time that 2-oxohistidine has been observed in a protein crystal structure.
A near atomic resolution structure of a Melanocarpus albomyces laccase.,Hakulinen N, Andberg M, Kallio J, Koivula A, Kruus K, Rouvinen J J Struct Biol. 2008 Apr;162(1):29-39. Epub 2007 Dec 28. PMID:18249560[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kiiskinen LL, Palonen H, Linder M, Viikari L, Kruus K. Laccase from Melanocarpus albomyces binds effectively to cellulose. FEBS Lett. 2004 Oct 8;576(1-2):251-5. PMID:15474046 doi:S0014579304010440
- ↑ Hakulinen N, Andberg M, Kallio J, Koivula A, Kruus K, Rouvinen J. A near atomic resolution structure of a Melanocarpus albomyces laccase. J Struct Biol. 2008 Apr;162(1):29-39. Epub 2007 Dec 28. PMID:18249560 doi:10.1016/j.jsb.2007.12.003
