1a3f
From Proteopedia
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PHOSPHOLIPASE A2 (PLA2) FROM NAJA NAJA VENOM
Overview
Three crystal forms of Naja naja naja phospholipase A2 were discovered, through random crystallization screening, including two heretofore, uncharacterized forms. The crystallization conditions for both of these, novel crystal forms are Ca(2+)-free whereas previously reported conditions, include Ca2+. One of the new crystal forms has a cubic lattice in the, space group P2(1)3 (a = b = c = 69.24 A), the other has an orthorhombic, lattice in the space group P2(1)2(1)2(1) (a = 67.22 A, b = 73.48 A, c =, 87.52 A) and a previously characterized crystal belong to the tetragonal, space group P4(3)2(1)2 (a = b = 88.6 A, c = 107.4 A). The structure from, the cubic crystal form has been determined to 1.8 A and refined to an, R-factor of 17% while the structure from the orthorhombic form has been, determined to 2.65 A and has been refined to an R-factor of 21%. The, determination of the cubic structure extends the resolution to which, structures of this molecule have been determined from 2.3 A to 1.8 A. The, two newly determined structures, in combination with the previously, determined structure, generate an informative structural ensemble from, which structural changes due to Ca2+, which is required for catalysis, and, the effect of crystal contacts on side-chain conformations and oligomeric, association can be inferred. Both of the newly determined structures, reveal a trimeric oligomer as observed in the tetragonal structure; this, appears to be a unique feature of the Naja naja naja enzyme.
About this Structure
1A3F is a Single protein structure of sequence from Naja naja. Active as Phospholipase A(2), with EC number 3.1.1.4 Structure known Active Sites: CAA, CAB and CAC. Full crystallographic information is available from OCA.
Reference
Structures of two novel crystal forms of Naja naja naja phospholipase A2 lacking Ca2+ reveal trimeric packing., Segelke BW, Nguyen D, Chee R, Xuong NH, Dennis EA, J Mol Biol. 1998 May 29;279(1):223-32. PMID:9636712
Page seeded by OCA on Mon Nov 5 15:18:58 2007
