Exonuclease

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E. coli exonuclease I complex with phosphate, glycerol and Mg+2 ion (PDB code 1fxx)

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Exonucleases (ExN) cleave nucleotides at the end of a polynucleotide chain^[1]. In E. coli there are ExN I to VIII. ExN were found in human and yeast as well.

ExN-I cleaves single-stranded DNA.
ExN-II is associated with DNA polymerase.
ExN-III cleaves double-stranded DNA.
ExN-IV cleavage results in nucleoside monophosphate.
ExN-V cleaves linear double-stranded DNA.
ExN-VIII requires a free 5’ OH group for reacting.

in E. coli exonuclease I (PDB code 1fxx).^[2] Water molecules shown as red spheres.

3D Structures of exonuclease

Updated on 14-February-2016

References

↑ Mukherjee D, Fritz DT, Kilpatrick WJ, Gao M, Wilusz J. Analysis of RNA exonucleolytic activities in cellular extracts. Methods Mol Biol. 2004;257:193-212. PMID:14770007 doi:http://dx.doi.org/10.1385/1-59259-750-5:193
↑ Breyer WA, Matthews BW. Structure of Escherichia coli exonuclease I suggests how processivity is achieved. Nat Struct Biol. 2000 Dec;7(12):1125-8. PMID:11101894 doi:10.1038/81978

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Exonuclease

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3D Structures of exonuclease

References

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